Description
The kit adopts a unique buffer system capable of removing humic acid from the soil sample as much as possible. It is provided with glass beads which can effectively crush various complex components in the soil sample and ensure the integrity of extracting genomic DNA from the soil. It is also suitable for the extraction of stool samples and intestinal microorganisms.
Required Reagents
Isopropanol, ethanol.
Features
■ Wide applicability: Suitable for extraction of various types of soil environmental samples such as flower bed soil, flower pot soil, farmland soil, mountain forest soil, silt, red soil, black soil, dust and so on. It is also suitable for the extraction of stool samples and intestinal microorganisms.
■ Convenient operation: The experimental operation can be completed in a relatively short time.
■ High purity: Combined with magnetic bead purification, the extracted DNA has high purity and can be directly used in downstream experiments
Applications
The DNA purified by the kit has few impurities and good integrity, and can be directly used in other downstream experiments of molecular biology such as PCR, enzyme digestion, etc.
Genomic DNA was extracted from 500 mg of garden soil by shaker and grinder respectively, using TIANGEN Magnetic Soil And Stool DNA Kit and relevant product from Supplier M. 5 μl of 100 μl eluate was loaded to 1% agarose gel electrophoresis. M: TIANGEN Maker D2000